Streptozotocin (STZ) 鏈脲佐菌素簡介：

產(chan)品名稱 ：鏈(lian)脲佐(zuo)菌素; 鏈(lian)氮霉素; 鏈(lian)脲菌素; 2-脫氧-2-(((甲基(ji)亞硝基(ji)氨(an)(an)基(ji))羰(tang)基(ji))-氨(an)(an)基(ji))-D-吡喃(nan)葡萄糖

英文名稱：STREPTOZOTOCIN ,2-desoxy-2-(3-methyl-3-nitrosoureido)-d-glucopyranose; 2-deoxy-2-(((methylnitrosoamino)carbonyl)amino)-d-glucose

產品介紹：鏈脲佐菌素(su)(su)是(shi)一(yi)種N-亞硝(xiao)基化合物(wu), 是(shi)一(yi)種氨基葡(pu)萄糖，是(shi)一(yi)種DNA烷基化試劑。作為胰島(dao)中一(yi)氧化氮供(gong)體,誘(you)導(dao)分泌(mi)胰島(dao)素(su)(su)的細(xi)(xi)(xi)胞(bao)(bao)死亡，產生糖尿病的動物(wu)模(mo)型(xing)。屬于強(qiang)有力(li)誘(you)導(dao)染色體斷裂的DNA甲(jia)基化劑。能通過GLUT2葡(pu)萄糖轉運(yun)蛋白（GLUT2 glucose transport protein）獨(du)自進入細(xi)(xi)(xi)胞(bao)(bao)。對表(biao)達GLUT2葡(pu)萄糖轉運(yun)子的神經內分泌(mi)腫瘤細(xi)(xi)(xi)胞(bao)(bao)株有細(xi)(xi)(xi)胞(bao)(bao)毒性。對胰腺胰島(dao)內胰島(dao)素(su)(su)誘(you)發的β-細(xi)(xi)(xi)胞(bao)(bao)具(ju)毒性。

性能

溶(rong)解性：鏈脲菌素溶(rong)于水，溶(rong)于乙(yi)醇

Streptozotocin (STZ) 鏈脲佐菌素用途： 生(sheng)(sheng)化(hua)實驗研究，廣泛應用于分子(zi)生(sheng)(sheng)物學，藥理(li)學等科研方(fang)面。一(yi)種含N-亞(ya)硝(xiao)(xiao)基(ji)的(de)(de)(de)化(hua)合(he)物，在(zai)(zai)(zai)(zai)胰腺胰島(dao)(dao)充當(dang)一(yi)氧化(hua)氮的(de)(de)(de)供體(ti)；誘導產生(sheng)(sheng)糖(tang)尿(niao)病(bing)(bing)動物模式的(de)(de)(de)胰島(dao)(dao)素分泌(mi)(mi)細(xi)(xi)胞的(de)(de)(de)死亡。高效DNA甲基(ji)化(hua)試劑，能誘發染(ran)色體(ti)斷裂(lie)。鏈(lian)(lian)脲(niao)菌素為Stre.achromogenes Uar.128產生(sheng)(sheng)的(de)(de)(de)亞(ya)硝(xiao)(xiao)脲(niao)類抗(kang)生(sheng)(sheng)素，它與(yu)脂溶性(xing)(xing)的(de)(de)(de)亞(ya)硝(xiao)(xiao)脲(niao)不同，在(zai)(zai)(zai)(zai)氯乙基(ji)處是一(yi)個甲基(ji)，在(zai)(zai)(zai)(zai)分子(zi)的(de)(de)(de)另一(yi)端(duan)是一(yi)個氨基(ji)糖(tang)。STZ可(ke)自行分解活(huo)潑的(de)(de)(de)甲基(ji)正(zheng)碳離子(zi)，與(yu)DNA呈鏈(lian)(lian)間(jian)交叉連結，從(cong)而(er)(er)使(shi)(shi)DNA烷(wan)化(hua)，但其(qi)烷(wan)化(hua)作(zuo)用比(bi)其(qi)他(ta)，而(er)(er)其(qi)代謝(xie)產物甲基(ji)亞(ya)硝(xiao)(xiao)脲(niao)的(de)(de)(de)烷(wan)化(hua)作(zuo)用較其(qi)STZ強3~4倍。STZ在(zai)(zai)(zai)(zai)體(ti)內(nei)可(ke)形成(cheng)異氰酸鹽。從(cong)而(er)(er)與(yu)核(he)酸蛋白(bai)結合(he)，抑制(zhi)DNA多聚(ju)酶活(huo)力，使(shi)(shi)受損的(de)(de)(de)DNA難于修復。在(zai)(zai)(zai)(zai)進行抗(kang)腫(zhong)(zhong)瘤研究過(guo)程中(zhong)發現，STZ可(ke)使(shi)(shi)鼠(shu)類的(de)(de)(de)血糖(tang)升高，在(zai)(zai)(zai)(zai)犬及猴可(ke)致糖(tang)尿(niao)病(bing)(bing)，且呈*性(xing)(xing)。STZ的(de)(de)(de)糖(tang)尿(niao)病(bing)(bing)作(zuo)用具有(you)種屬差異性(xing)(xing)，在(zai)(zai)(zai)(zai)豚鼠(shu)不引起，在(zai)(zai)(zai)(zai)人亦不引起。其(qi)致糖(tang)尿(niao)病(bing)(bing)機(ji)制(zhi)主要(yao)是由于胰島(dao)(dao)細(xi)(xi)胞中(zhong)菸酰胺腺嘌呤（DNA）含量減(jian)少，STZ分子(zi)中(zhong)的(de)(de)(de)葡萄(tao)糖(tang)基(ji)可(ke)使(shi)(shi)STZ進入胰島(dao)(dao)β細(xi)(xi)胞，引起β細(xi)(xi)胞核(he)內(nei)形態變化(hua)，使(shi)(shi)其(qi)染(ran)色體(ti)凝(ning)集、伸長和濃縮(suo)。對表達(da)GLUT2葡萄(tao)糖(tang)轉運體(ti)（GLUT2 glucose transporter）的(de)(de)(de)神經內(nei)分泌(mi)(mi)腫(zhong)(zhong)瘤細(xi)(xi)胞系具細(xi)(xi)胞毒性(xing)(xing)。

醫學研究上鏈脲佐菌素用于誘導Type 1 糖尿病。
儲(chu)存條件 ：2～8℃

Sigma相關資料如下

Product: ：Streptozocin

Molecular Weight: 265.2

Molecular Formula: C8H15N3O7

Code: 0130

CAS：18883-66-4

PHYSICAL DESCRIPTION:

Appearance: White to yellow powder

Molecular formula: C8H15N3O7

Molecular weight: 265.2

Melting point: Decomposes at 115EC if anhydrous.1 Although Sigma does not determine a value, water

content should be # 3%.2

EmM(228nm) = 6.36 (ethanol)

Optical rotation: +39E(equilibrium of a, ? anomers in H2O, 25EC)1

Please consult the Material Safety Data Sheet about the properties of this material as a potential carcinogen, mutagen and toxic chemical.

STORAGE / STABILITY AS SUPPLIED:

If the product is stored frozen and protected from moisture and air, it is stable for approximay 2 years.(After 12 year, a sample changed from 94.9% a-anomer to 94.7%, as measured by HPLC.)2

SOLUBILITY / SOLUTION STABILITY:

Streptozotocin is soluble in water, the lower alcohols and in ketones. This product dissolves in water at 50mg/mL to give a light yellow solution, from clear to slightly hazy. Aqueous solutions rapidly undergo mutarotation to an equilibrium mixture of alpha- and beta-anomers.

STREPTOZOTOCIN MIXED ANOMERS

Sigma No. S0130

SOLUBILITY / SOLUTION STABILITY:

Maximum solution stability is at pH 4, with stability decreasing rapidly at higher or lower pH. Freshly prepared solutions are clear and have a light straw color. On standing, they take on a yellow to brown color and effervesce, indicating decomposition.2,3 Solutions should be prepared just before use,since the product is unstable.

GENERAL REMARKS:

This product is an antineoplastic antibiotic produced by the growth of a Streptomyces achromogenes variant or by synthesis. It may affect glucose metabolism. It is used mainly in the treatment of pancreatic (isletcell) tumors.4 Burcelin et al. used intravenous injection of streptozotocin in rats at a dose of 65 mg/kg body weight to induce diabetes (using cold 0.1 M citrate buffer pH 4.5).5 In rats and dogs, diabetes was induced using intravenous dosage of 50 mg/kg (using 1-2% w/v solutions in saline buffered with citrate dextrosesolution at pH 5.0).3 It has been used for the treatment of malignant insulinoma; very precise assays for thisdrug have been developed.6

Streptozotocin does not cross the blood-brain barrier, but its metabolites are found in cerebral spinal fluid.4 Its biological half-life in cell culture medium was shown to be approximay 19 minutes.7

The antileukemic effects of streptozotocin and its analogs have been reported.8 Streptozotocin has been shown to be a potent methylating agent that reacts with DNA in vitro to form methylated purines.9 A reviewarticle addressed a number of antineoplastic antibiotics, including streptozotocin.10 A useful handbookoffered several references for use in animal studies.11

REFERENCES:

1. Merck Index, 12th ed., #8991 (1996).

2. Sigma quality control or supplier information.

3. Rakieten, N. et al., Cancer Chemother. Reports, No. 29, 91-98 (1963).

4. Martindale: The Extra Pharmacopoeia, 29th ed., (Pharmaceutical Press, 1989), p. 649.

5. Burcelin, R. et al., Biochem. J., 291, 109-113 (1993).

6. Oles, P.J., J. Pharmaceutical Sci., 67 (9), 1300 (1978).

7. Jensen, E.M. et al., J. Natl. Cancer Inst., 59, 941-944 (1977).

8. Bhuyan, B.K. et al., Cancer Chemother. Reports, Part 1, 45 (6), 709-720 (1972).

9. Bennett, R.A. and Pegg, A.E., Cancer Research, 41, 2756-2790 (1981).

10. Cheng, C.C. and K.-Y. Zee-Cheng, J. Pharmaceutical Sci., 61, 485-501 (1972).

11. Drug Dosage in Laboratory Animals: A Handbook , 3rd ed., R.E. Borchard et al., eds. (CRC Press,

1992).

Sigma warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particularuse. Additional terms and conditions may apply. Please see reverse side of the invoice orpacking slip.

Streptozotocin (STZ) 鏈脲佐菌素訂購信息：

上(shang)海(hai)懋康(kang)生(sheng)物(wu)科技有(you)限公司是一家涉足于生(sheng)命(ming)科學(xue)和(he)生(sheng)物(wu)技術領(ling)(ling)域(yu)研(yan)究的(de)試劑(ji)、儀器和(he)實驗室消耗品(pin)與(yu)實驗服務工作，主(zhu)要(yao)從事細胞生(sheng)物(wu)學(xue)、植物(wu)學(xue)、分子(zi)生(sheng)物(wu)學(xue)、免疫學(xue)、生(sheng)物(wu)化學(xue)、蛋白組學(xue)。生(sheng)物(wu)制藥與(yu)診斷試劑(ji)研(yan)發生(sheng)產(chan)等領(ling)(ling)域(yu)。 本公司秉承“以人(ren)為(wei)本，以誠為(wei)信(xin)、合同守信(xin)"的(de)經營理(li)念。堅持(chi)"品(pin)質保障"的(de)原則為(wei)廣大客戶提(ti)供(gong)優質產(chan)品(pin)。

Streptozotocin （STZ） 鏈脲佐菌素 糖(tang)尿病(bing)建模(mo)

Streptozotocin （STZ） 鏈脲佐菌素(su) 糖尿病建模